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Tackle your drug interaction studies, inhibition studies, substrate studies, and pharmacokinetic studies with our Vesicular Transport Assays:
Human drug efflux transporters (ABC transporters) are transiently overexpressed in human embryonic kidney (HEK293) cells. Inside-out membrane vesicles are prepared from these cells and used for high-throughput applications. The transporters translocate substrates into membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radiolabel, fluorescent or LC-MS detection. Cell4Pharma delivers high quality large uniform batches with guaranteed transport ratios.
Complete list of all available transporters
Vesicular Transport Assays One-pager (PDF)
Vesicular Transport Assay General Protocol
Our vesicles have an unmet dynamic range compared to others in the market.
PgP
Inside-out membrane vesicles isolated from HEK293 cells. PgP vesicles with an ATP/AMP ratio >5 are readily available from Cell4Pharma. PgP mediated convallatoxin (digoxin analogue) transport:
Name: MDR1, multidrug resistance protein 1, permeability glycoprotein, P-gp, ATP-binding cassette sub-family B member 1, ABCB1.
Diseases: – .
PgP substrates: convallatoxin, digoxin, N-methyl-quinidine (NMQ), colchicine, tacrolimus, quinidine, etoposide, doxorubicin, etc. PgP is present in: Intestine, liver, kidney, blood-brain barrier, many tumor cells.
In stock: human Pgp.
Available: mouse and rat Pgp.
For ordering information or product quotes please contact us here.
Data sheet MDR1
BSEP
Inside-out membrane vesicles isolated from HEK293 cells. BSEP vesicles with an ATP/AMP ratio >10 are readily available from Cell4Pharma.
BSEP mediated TCA transport:
Name: BSEP, Bile Salt Export Pump, ATP-binding cassette sub-family B member 11, ABCB11.
Diseases: inherited and drug-induced cholestasis.
BSEP substrates: taurocholate, pravastatin, taurolitocholyl 3-sulfate, conjugated monovalent bile salts.
BSEP is present in: canalicular plasma membrane of hepatocytes.
In stock: human, cat, and dog BSEP.
Available: mouse and rat BSEP.
For ordering information or product quotes please contact us here.
Data sheet BSEP
MRP1
Inside-out membrane vesicles isolated from HEK293 cells. MRP1 vesicles with an ATP/AMP ratio >5 are readily available from Cell4Pharma.
MRP1 mediated E217bG transport:
Name: MRP1, multidrug resistance associated protein 1, ATP-binding cassette sub-family C member 1, ABCC1.
Diseases: – .
MRP1 substrates: E217bG, GSSG, saquinavir, ritonavir, methotrexate, daunorubicin, leukotrienes, prostaglandines, folic acid, etc.
MRP1 is present in: lung, testis, kidney, blood-brain barrier, skeletal and cardiac muscles, placenta, and macrophages.
In stock: human MRP1.
Available: mouse and rat MRP1.
For ordering information or product quotes please contact us here.
Data sheet MRP1
MRP2
Inside-out membrane vesicles isolated from HEK293 cells. MRP2 vesicles with an ATP/AMP ratio >20 are readily available from Cell4Pharma.
MRP2 mediated CDCF transport:
Name: MRP2, multidrug resistance associated protein 2, cMOAT, canalicular multispecific organic anion transporter, ATP-binding cassette sub-family C member 2, ABCC2.
Diseases: Dubin-Johnson syndrome.
MRP2 substrates: CDCF, E217bG, methotrexate, etoposide, bilirubin conjugates, etc.
MRP2 is present in: liver, kidney, blood-brain-barrier, intestine, colon, bronchi and placenta.
In stock: human MRP2.
Available: mouse and rat MRP2.
For ordering information or product quotes please contact us here.
Data sheet MRP2
MRP3
Inside-out membrane vesicles isolated from HEK293 cells. MRP3 vesicles with an ATP/AMP ratio >10 are readily available from Cell4Pharma.
MRP3 mediated E217bG transport:
Name: MRP3, multidrug resistance associated protein 3, ATP-binding cassette sub-family C member 3, ABCC3.
Diseases: – .
MRP3 substrates: E217bG, estradiol-17b-glucuronide, bile salts, methotrexate, vincristine, acetaminophen-glucuronide, etc.
MRP3 is present in: liver, gallbladder, pancreas, intestine, colon, kidney, spleen and adrenal cortex .
In stock: human MRP3.
Available: mouse and rat MRP3.
For ordering information or product quotes please contact us here.
Data sheet MRP3
MRP4
Inside-out membrane vesicles isolated from HEK293 cells. MRP4 vesicles with an ATP/AMP ratio >5 are readily available from Cell4Pharma.
MRP4 mediated DHEAS transport:
Name: MRP4, multidrug resistance associated protein 4, MOATB, ATP-binding cassette sub-family C member 4, ABCC4.
Diseases: – .
MRP4 substrates: DHEAS, E217bG, taurocholic acid, urate, ritonavir, methotrexate, cAMP, etc.
MRP4 is present in: kidney, prostate, liver, pancreas and choroid plexus basolaterally and brain capillary endothelial cells apically.
In stock: human MRP4.
Available: mouse and rat MRP4.
For ordering information or product quotes please contact us here.
Data sheet MRP4
MRP5
Inside-out membrane vesicles isolated from HEK293 cells. MRP5 vesicles with an ATP/AMP ratio >3 are readily available from Cell4Pharma.
MRP5 mediated CDCF transport:
Name: MRP5, multidrug resistance associated protein 5, MOATC, ATP-binding cassette sub-family C member 5, ABCC5.
Diseases: – .
MRP5 substrates: CDCF, methotraxate, cAMP, cGMP, folate, rouvastatin, etc.
MRP5 is present in: liver, lung, urethra, blood-brain-barrier, choroid plexus, skeletal muscle, heart, and placenta.
In stock: human MRP5.
Available: mouse and rat MRP5.
For ordering information or product quotes please contact us here.
Data sheet MRP5
MRP8
Inside-out membrane vesicles isolated from HEK293 cells. MRP8 vesicles with an ATP/AMP ratio >3 are readily available from Cell4Pharma.
MRP8 mediated DHEAS transport:
Name: MRP8, ATP-binding cassette sub-family C member 11, ABCC11.
Diseases: – .
MRP8 substrates: DHEAS, 5-fluoro-2′-deoxyuridine 5′-monophosphate (FdUMP), earwax, etc.
MRP8 is present in: brain, breasts, lungs, liver, kidney, placenta, prostate, testes, gastrointestinal tract tissues, gastric mucosa, and apocrine glands.
In stock: human MRP8.
Available: mouse and rat MRP8.
For ordering information or product quotes please contact us here.
Data sheet MRP8
BCRP
Inside-out membrane vesicles isolated from HEK293 cells. BCRP vesicles with an ATP/AMP ratio >10 are readily available from Cell4Pharma.
BCRP mediated E1S transport:
Name: BCRP, breast cancer resistance protein, ATP-binding cassette sub-family G member 2, ABCG2.
Diseases: – .
BCRP substrates: E1S, estrone-3-sulfate, methotrexate, irinotecan, pantoprazole, rosuvastatin, topotecan, chlorothiazide etc.
BCRP is present in: placenta, stem cells, prostate, small intestine, brain, colon, liver, mammary gland and kidney.
In stock: human BCRP.
Available: mouse and rat BCRP.
For ordering information or product quotes please contact us here.
Data sheet BCRP
Our vesicles have an unmet dynamic range compared to others in the market.
PGP
Inside-out membrane vesicles isolated from HEK293 cells. PgP vesicles with an ATP/AMP ratio >5 are readily available from Cell4Pharma. PgP mediated convallatoxin (digoxin analogue) transport:
Name: MDR1, multidrug resistance protein 1, permeability glycoprotein, P-gp, ATP-binding cassette sub-family B member 1, ABCB1.
Diseases: – .
PgP substrates: convallatoxin, digoxin, N-methyl-quinidine (NMQ), colchicine, tacrolimus, quinidine, etoposide, doxorubicin, etc. PgP is present in: Intestine, liver, kidney, blood-brain barrier, many tumor cells.
In stock: human Pgp.
Available: mouse and rat Pgp.
For ordering information or product quotes please contact us here.
BSEP
Inside-out membrane vesicles isolated from HEK293 cells. BSEP vesicles with an ATP/AMP ratio >10 are readily available from Cell4Pharma.
BSEP mediated TCA transport:
Name: BSEP, Bile Salt Export Pump, ATP-binding cassette sub-family B member 11, ABCB11.
Diseases: inherited and drug-induced cholestasis.
BSEP substrates: taurocholate, pravastatin, taurolitocholyl 3-sulfate, conjugated monovalent bile salts.
BSEP is present in: canalicular plasma membrane of hepatocytes.
In stock: human, cat, and dog BSEP.
Available: mouse and rat BSEP.
For ordering information or product quotes please contact us here.
MRP1
Inside-out membrane vesicles isolated from HEK293 cells. MRP1 vesicles with an ATP/AMP ratio >5 are readily available from Cell4Pharma.
MRP1 mediated E217bG transport:
Name: MRP1, multidrug resistance associated protein 1, ATP-binding cassette sub-family C member 1, ABCC1.
Diseases: – .
MRP1 substrates: E217bG, GSSG, saquinavir, ritonavir, methotrexate, daunorubicin, leukotrienes, prostaglandines, folic acid, etc.
MRP1 is present in: lung, testis, kidney, blood-brain barrier, skeletal and cardiac muscles, placenta, and macrophages.
In stock: human MRP1.
Available: mouse and rat MRP1.
For ordering information or product quotes please contact us here.
MRP2
Inside-out membrane vesicles isolated from HEK293 cells. MRP2 vesicles with an ATP/AMP ratio >20 are readily available from Cell4Pharma.
MRP2 mediated CDCF transport:
Name: MRP2, multidrug resistance associated protein 2, cMOAT, canalicular multispecific organic anion transporter, ATP-binding cassette sub-family C member 2, ABCC2.
Diseases: Dubin-Johnson syndrome.
MRP2 substrates: CDCF, E217bG, methotrexate, etoposide, bilirubin conjugates, etc.
MRP2 is present in: liver, kidney, blood-brain-barrier, intestine, colon, bronchi and placenta.
In stock: human MRP2.
Available: mouse and rat MRP2.
For ordering information or product quotes please contact us here.
MRP3
Inside-out membrane vesicles isolated from HEK293 cells. MRP3 vesicles with an ATP/AMP ratio >10 are readily available from Cell4Pharma.
MRP3 mediated E217bG transport:
Name: MRP3, multidrug resistance associated protein 3, ATP-binding cassette sub-family C member 3, ABCC3.
Diseases: – .
MRP3 substrates: E217bG, estradiol-17b-glucuronide, bile salts, methotrexate, vincristine, acetaminophen-glucuronide, etc.
MRP3 is present in: liver, gallbladder, pancreas, intestine, colon, kidney, spleen and adrenal cortex .
In stock: human MRP3.
Available: mouse and rat MRP3.
For ordering information or product quotes please contact us here.
MRP4
Inside-out membrane vesicles isolated from HEK293 cells. MRP4 vesicles with an ATP/AMP ratio >5 are readily available from Cell4Pharma.
MRP4 mediated DHEAS transport:
Name: MRP4, multidrug resistance associated protein 4, MOATB, ATP-binding cassette sub-family C member 4, ABCC4.
Diseases: – .
MRP4 substrates: DHEAS, E217bG, taurocholic acid, urate, ritonavir, methotrexate, cAMP, etc.
MRP4 is present in: kidney, prostate, liver, pancreas and choroid plexus basolaterally and brain capillary endothelial cells apically.
In stock: human MRP4.
Available: mouse and rat MRP4.
For ordering information or product quotes please contact us here.
MRP5
Inside-out membrane vesicles isolated from HEK293 cells. MRP5 vesicles with an ATP/AMP ratio >3 are readily available from Cell4Pharma.
MRP5 mediated CDCF transport:
Name: MRP5, multidrug resistance associated protein 5, MOATC, ATP-binding cassette sub-family C member 5, ABCC5.
Diseases: – .
MRP5 substrates: CDCF, methotraxate, cAMP, cGMP, folate, rouvastatin, etc.
MRP5 is present in: liver, lung, urethra, blood-brain-barrier, choroid plexus, skeletal muscle, heart, and placenta.
In stock: human MRP5.
Available: mouse and rat MRP5.
For ordering information or product quotes please contact us here.
MRP8
Inside-out membrane vesicles isolated from HEK293 cells. MRP8 vesicles with an ATP/AMP ratio >3 are readily available from Cell4Pharma.
MRP8 mediated DHEAS transport:
Name: MRP8, ATP-binding cassette sub-family C member 11, ABCC11.
Diseases: – .
MRP8 substrates: DHEAS, 5-fluoro-2′-deoxyuridine 5′-monophosphate (FdUMP), earwax, etc.
MRP8 is present in: brain, breasts, lungs, liver, kidney, placenta, prostate, testes, gastrointestinal tract tissues, gastric mucosa, and apocrine glands.
In stock: human MRP8.
Available: mouse and rat MRP8.
For ordering information or product quotes please contact us here.
BCRP
Inside-out membrane vesicles isolated from HEK293 cells. BCRP vesicles with an ATP/AMP ratio >10 are readily available from Cell4Pharma.
BCRP mediated E1S transport:
Name: BCRP, breast cancer resistance protein, ATP-binding cassette sub-family G member 2, ABCG2.
Diseases: – .
BCRP substrates: E1S, estrone-3-sulfate, methotrexate, irinotecan, pantoprazole, rosuvastatin, topotecan, chlorothiazide etc.
BCRP is present in: placenta, stem cells, prostate, small intestine, brain, colon, liver, mammary gland and kidney.
In stock: human BCRP.
Available: mouse and rat BCRP.
For ordering information or product quotes please contact us here.
Vesicular Transport Assays
Cell4Pharma delivers high quality large uniform batches with guaranteed transport ratios. Transporters in the list below can be shipped directly after ordering. Transporters not in this list (including polymorphisms and mutations) can be custom made within 2 months.
| Transporter | Gene name | Species | Substrates | Inhibitors | Test substrate | AMP/ATP ratio |
|---|---|---|---|---|---|---|
| P-glycoprotein / MDR1 | ABCB1 | human | convallatoxin, digoxin, N-methyl-quinidine (NMQ), colchicine, tacrolimus, quinidine, etoposide, doxorubicin ao | verapamil, cyclosporin, tariquidar, PSC833, ao | convallatoxin | >5 |
| BSEP, bile salt export pump | ABCB11 | human | taurocholate, pravastatin, taurolitocholyl 3-sulfate, conjugated monovalent bile salts, ao | cyclosporine A, rifampicin, glibenclamide ao | TCA | >20 |
| BSEP, bile salt export pump | ABCB11 | rat | TCA | >10 | ||
| BSEP, bile salt export pump | ABCB11 | dog | TCA | >10 | ||
| BSEP, bile salt export pump | ABCB11 | cat | TCA | >10 | ||
| MRP1, multidrug resistance associated protein 1 | ABCC1 | human | E217bG, GSSG, saquinavir, ritonavir, methotrexate, daunorubicin, leukotrienes, prostaglandines, folic acid, ao | 17b-Estradiol-D-17b-Glucuronide | >5 | |
| MRP2, multidrug resistance associated protein 2 | ABCC2 | human | CDCF, E217bG, methotrexate, etoposide, bilirubin conjugates, ao | 5(6)-carboxy-2,’7′-dichlorofluorescein | >20 | |
| MRP2, multidrug resistance associated protein 2 | ABCC2 | rat | CDCF | >20 | ||
| MRP3, multidrug resistance associated protein 3 | ABCC3 | human | E217bG, estradiol-17b-glucuronide, bile salts, methotrexate, vincristine, acetaminophen-glucuronide, ao | 17b-Estradiol-D-17b-Glucuronide | >15 | |
| MRP3, multidrug resistance associated protein 3 | ABCC3 | rat | 17b-Estradiol-D-17b-Glucuronide | >5 | ||
| MRP4, multidrug resistance associated protein 4 | ABCC4 | human | DHEAS, E217bG, taurocholic acid, urate, ritonavir, methotrexate, cAMP, ao | Dehydroepiandrosterone sulfate | >5 | |
| MRP4, multidrug resistance associated protein 4 | ABCC4 | rat | p-aminohippuric acid | >2 | ||
| MRP5, multidrug resistance associated protein 5 | ABCC5 | human | CDCF, methotraxate, cAMP, cGMP, folate, rouvastatin, ao | 5(6)-carboxy-2,’7′-dichlorofluorescein | >3 | |
| MRP8, multidrug resistance associated protein 8 | ABCC11 | human | DHEAS, 5-fluoro-2′-deoxyuridine 5′-monophosphate (FdUMP), earwax, ao | Dehydroepiandrosterone sulfate | >3 | |
| BCRP | ABCG2 | human | estrone-3-sulfate (E3S), methotrexate, irinotecan, pantoprazole, rosuvastatin, topotecan, chlorothiazide, ao | Estrone-sulfate | >10 |
Cellular Transport Assays
Cellular Transport Assays are under development with promising data. Current development includes transferring ready to use product to our customers upon request. Alternatively, contract research activities are available upon request.
| Transporter | Gene name | Species | Substrates | Inhibitors | Test substrate | Transport ratio |
|---|---|---|---|---|---|---|
| ABCA1 | ABCA1 | human | Cholesterol | Cholesterol | ||
| ABCG1 | ABCG1 | human | Cholesterol | Cholesterol | ||
| GLUT1 | SLC2A1 | human | D-Glucose | Deoxy-D-Glucose | ||
| GLUT2 | SLC2A2 | human | D-Glucose | Deoxy-D-Glucose | >3 | |
| GLUT5 | SLC2A5 | human | D-Glucose | Deoxy-D-Glucose | >3 | |
| SGLT1 | SLC5A1 | human | D-Glucose | AMG | >20 | |
| SGLT2 | SLC5A2 | human | D-glucose | AMG | ||
| SGLT1 | SLC5A1 | dog | D-glucose | AMG | ||
| DAT, Dopamine transporter | SLC6A3 | human | Dopamine, Serotonin, ao | Dopamine | ||
| SERT, Serotonin transporter | SLC6A4 | human | Serotonin, Dopamine, ao | Serotonin | ||
| LAT2 | SLC7A8 (optional in combination with 4F2) | human | L-Alanine | |||
| NTCP | SLC10A1 | human | Taurocholate, bile salts, DHEAS, Rosuvastatin, atorvastatin, pitavastatin, fluvastatin | Taurocholate (TCA) | ||
| PEPT1 | SLC15A1 | human | Gly-Sar (Glycyl-sarcosine) | >20 | ||
| PEPT2 | SLC15A2 | human | Gly-Sar (Glycyl-sarcosine) | |||
| OCT1 | SLC22A1 | human | Acyclovir, Amiloride, Fenoterol, Furamidine, Ganciclovir, Glycopyrrolate, Ipratropium, Metformin, N-methylpyridinium, Prostaglandin E2, Ranitidine, Tetraethylammonium, ao |
MDMA, Atropine, Cimetidine, Clonidine, Diphenhydramine, Ketamine, Midazolam, Quinidine, Verapamil, ao | Metformin | >3 |
| OCT2 | SLC22A2 | human | ASP+, Amiloride, Cimetidine, Dopamine, Epinephrine, Metformin, N-methylpyridinium, Prostaglandin E2, Serotonin, Tetraethylammonium, ao | MDMA, Atropine, Berberine, Cimetidine, Flurazepam, Imipramine, Quinine, Verapamil, ao | Metformin | >4 |
| OCT3 | SLC22A3 | human | Berberine, Epinephrine, Etilefrine, Histamine, Metformin, N-methylpyridinium, Norepinephrine, ao | MDMA, Atropine, Berberine, Imipramine, Phenoxy-benzamine, Quinidine, Tetraethylammonium, ao | Metformin | >7 |
| OCTN1 | SLC22A4 | human | L-Carnitine | >2 | ||
| OCTN2 | SLC22A5 | human | L-Carnitine | >40 | ||
| OAT1 | SLC22A6 | human | 6-carboxyfluorescein, Adefovir, Cidofovir, Olmesartan, Para-aminohippurate, Prostaglandin E2, Uric acid | Acetominophen, Adefovir, Betamipron, Chlorothiazide, Diclofenac, Furosemide, Ibuprofen, Indomethacin, Probenecid, ao | CDCF | |
| OAT2 | SLC22A7 | human | Bumetanide, Cefoperazone, Cephalothin, Chlorothiazide, Diclofenac, Furosemide, Ibuprofen, Indomethacin, Ketoprofen, Mefenamic acid, Naproxen, Phenacetin, Piroxicam, Probenecid, Sulindac, ao |
MDMA, Atropine, Cimetidine, Clonidine, Decynium-22, Diphenhydramine, Furamidine, Memantine, Phencyclidine, Phenformin, Quinidine, Repaglinide, Verapamil, ao |
PAH | |
| OAT3 | SLC22A8 | human | Bumetanide, Cefaclor, Cimetidine, Cortisol, Estrone-3-sulfate, Furosemide, Methotrexate, Pravastatin, Prostaglandin E2, Rosuvastatin, Tetracycline, Uric acid, ao | Bumetanide, Candesartan, Ethacrynic acid, Furosemide, Ibuprofen, Naproxen, Olmesartan, Pratosartan, Quinapril, Sulindac, Valsartan, ao | Estrone sulfate | |
| OAT4 | SLC22A11 | human | ||||
| OAT5 | SLC22A10 | human | ||||
| URAT1 | SLC22A12 | human | Oxypurinol, Uric acid | Benzbromarone, Fenofibric acid, Losartan, Morin, Pratosartan, Probenecid, Sulfinpyrazone, Telmisartan | Uric acid | |
| ENT4 | SLC29A4 | human | ||||
| MATE1 | SLC47A1 | human | Cimetidine, Estrone sulfate, Ganciclovir, Metformin, Paraquat, Tetraethylammonium (TEA), ao | Chloroquine, Cimetidine, Mixantrone, Pyrimethamine, Quinidine, Verapamil, oa | Metformin | |
| MATE2K | SLC47A2 | human | Cimetidine, Estrone sulfate, Ganciclovir, Metformin, Tetraethylammonium (TEA), Topotecan, ao | Cimetidine, Pyrimethamine, Quinidine, Verapamil, oa | Metformin | |
| OATP1A2 | SLCO1A1 | human | Taurocholic acid, Ursodeoxycholic acid, cholic acid, DHEAS, ao | Statins, Ouabain, Imatinib, Fexofenadine, Atenolol, Testosterone, Naloxone, Quinidine, Rifampicin, ao | Estrone sulfate | |
| OATP1B1 | SLCO1B1 available *1B, *4, *5, *15 | human | Atorvastatin, Bilirubin, Bosentan, Bromsulphthalein (BSP), Cholate, Cholyl-glycylamido-fluorescein (CGamF), Cholyl taurine, Dehydroepiandrosterone sulfate, Estradiol-17beta-glucuronide, Estrone 3-sulfate, Fluo-3, Fluvastatin, Methotrexate, Olmesartan, Pravastatin, Rifampicin, Rosuvastatin, Tauroursodeoxycholate, Valsartan, ao |
Cyclosporine, Digoxin, Fenofibrate, Gemfibrozil, Lopinavir, MK-571, Troglitazone, ao | 17b-Estradiol-D-17b-Glucuronide | |
| OATP1B3 | SLCO1B3 | human | Amantinin, Bilirubin, Bosentan, Bromsulphthalein (BSP), Cholecystokinin, Cholyl-glycylamido-fluorescein (CGamF), Digoxin, Estradiol-17beta-glucuronide, Estrone 3-sulfate, Fexofenadine, Fluo-3, Fluvastatin, Glycoursodeoxycholate, Methotrexate, Olmesartan, Paclitaxel, Rifampicin, Rosuvastatin, Taurocholate, Tauroursodeoxycholate, Telmisartan, Triiodothyronine, Valsartan, ao |
Cyclosporine, Darunavir, Lithocholate, Lopinavir, Mifepristone, MK-571, Paclitaxel, Penicillin G, Probenecid, Rifampicin, Ritonavir, ao | 17b-Estradiol-D-17b-Glucuronide, TCA | |
| OATP2B1 | SLCO2B1 | human | Atorvastatin, Bosentan, Bromsulphthalein (BSP), Estrone 3-sulfate, Fexofenadine, Fluvastatin, Glyburide, Pitavastatin, Pravastatin, Rosuvastatin, Taurocholate, Telmisartan glucuronide, ao |
Cerivastatin, Cyclosporine, Estrone, Gemfibrozil, Indinavir, MK-571, Montelukast, Paclitaxel, Repaglinide, Rifampicin, Rifamycin SV, Ritonavir, Rosiglitazone, Saquinavir, Silibinin dihemisuccinate, Simvastatin, ao |
DHEAS | >10 |
Vesicular Transport Assays
Drug efflux transporters (ABC transporters) are transiently overexpressed in human embryonic kidney (HEK293) cells. Inside-out membrane vesicles are prepared from these cells and used for high-throughput applications. The transporters translocate various compounds into membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radiolabel, fluorescent or LC-MS detection. Cell4Pharma delivers high quality large uniform batches with guaranteed transport ratios. Transporters in the list below can be shipped directly after ordering. Transporters not in this list (including polymorphisms and mutations) can be custom made within 2 months.
Cellular Transport Assays
Cellular Transport Assays are under development with promising data. Current development includes transferring ready to use product to our customers upon request. Alternatively, contract research activities are available upon request.
Our vesicles have an unmet dynamic range.
Get more information
Our pricing is tailored to fit your needs
Request a quote
We will get back to you as soon as possible.
Testimonials
Because the cell line is robust in culture, the research leads to reliable results. As far as I am concerned, there is currently no kidney epithelial cell line available with an equally good predictive value for the human kidney.
The ciPTEC mimics primary renal cells, but you can culture them for a long time without losing their properties. The expression of transporters gives a more accurate response to drugs and toxins. The cells grow fast and are easy to handle.
ciPTEC is a well-accepted in vitro model to screen compounds for their DIKI potential.