Researchers we empower:
Empowering CRO Research
Stay ahead of the competition by using our highly accurate and stable human renal cell lines
Win more projects and grow your clients
To stay ahead of the competition requires you to provide the best service. We help CRO’s to provide a cutting edge service with our ciPTEC cell lines and expertise. It allows you to showcase more accurate and stable results to your clients. Working with compounds that show to be toxic in later stages of clinical research is an unfortunate waste of work and investment. Therefore, it is essential for CRO’s to increase the chance of success as early as possible. It will stimulate the relationship with your clients and help grow their accounts. We have demonstrated this early detection process with AstraZeneca. A validation set of 62 drugs, tested across a relevant concentration range compared to their exposure in humans, to develop a model that integrates multi-parametric data and drug exposure information, which identified most proximal tubular toxic drugs tested (sensitivity 75%) without any false positives (specificity 100%).
Deliver cutting edge research
Delivering more accurate research with our cell lines. ciPTEC is the basis for a range of cell-based assays. More than 10 years of research and partnership with the pharmaceutical industry have led to a range of cell-based assays based on ciPTEC. Next to drug interaction studies, IC50 determinations, toxicity studies, gene expression analysis, miRNA biomarker screenings, ciPTEC allows the detection of specific biomarkers upon toxicant exposures. Furthermore, the introduction of a temperature sensitive gene led to the unlimited proliferation with maintained human proximal tubular characteristics. As a result, ciPTEC is a robust and stable cell line that can be implemented in high throughput screening and high volume research for your clients.
Accurate and reproducible renal toxicity research
The functional drug transporters and endocytic apparatus lead to a realistic cellular response to toxicant exposures. This evolved insight on the detailed renal toxic mechanisms utilizes a higher chance to reach market successfully. Cell4Pharma’s ciPTEC technology is based on a human renal cell line with unlimited proliferation capacity. Our cells have stable drug transport functionality for at least 20 passages. When creating your own stock, this means your assays will provide the same data, over and over again. Our experts in the field of pharmacology and toxicology can support you to elucidate the underlying mechanism of renal adverse effects observed in your lead compounds by developing cell-based assays based on our ciPTEC technology.
Empowering CRO’s
Stay ahead of the competition by using our highly accurate and stable human renal cell lines
Win more projects and grow your clients
To stay ahead of the competition requires you to provide the best service. We help CRO’s to provide a cutting edge service with our ciPTEC cell lines and expertise. It allows you to showcase more accurate and stable results to your clients. Working with compounds that show to be toxic in later stages of clinical research is an unfortunate waste of work and investment. Therefore, it is essential for CRO’s to increase the chance of success as early as possible. It will stimulate the relationship with your clients and help grow their accounts. We have demonstrated this early detection process with AstraZeneca. A validation set of 62 drugs, tested across a relevant concentration range compared to their exposure in humans, to develop a model that integrates multi-parametric data and drug exposure information, which identified most proximal tubular toxic drugs tested (sensitivity 75%) without any false positives (specificity 100%).
Deliver cutting edge research
Delivering more accurate research with our cell lines. ciPTEC is the basis for a range of cell-based assays. More than 10 years of research and partnership with the pharmaceutical industry have led to a range of cell-based assays based on ciPTEC. Next to drug interaction studies, IC50 determinations, toxicity studies, gene expression analysis, miRNA biomarker screenings, ciPTEC allows the detection of specific biomarkers upon toxicant exposures. Furthermore, the introduction of a temperature sensitive gene led to the unlimited proliferation with maintained human proximal tubular characteristics. As a result, ciPTEC is a robust and stable cell line that can be implemented in high throughput screening and high volume research for your clients.
Accurate and reproducible renal toxicity research
The functional drug transporters and endocytic apparatus lead to a realistic cellular response to toxicant exposures. This evolved insight on the detailed renal toxic mechanisms utilizes a higher chance to reach market successfully. Cell4Pharma’s ciPTEC technology is based on a human renal cell line with unlimited proliferation capacity. Our cells have stable drug transport functionality for at least 20 passages. When creating your own stock, this means your assays will provide the same data, over and over again. Our experts in the field of pharmacology and toxicology can support you to elucidate the underlying mechanism of renal adverse effects observed in your lead compounds by developing cell-based assays based on our ciPTEC technology.
ciPTEC Cell Lines Characteristics
Robust data
The stable transfection and maintained epithelial characteristics lead to a cell line that will produce very robust data. Our validation studies demonstrated robust data for more than 20 passages.
Transferable between laboratories
Our ciPTEC technology is a result of more than 10 years of research in world-wide collaborations. We have successfully transferred our protocols to collaborating laboratories.
Intact drug transporter functionality
Using fluorescent probes specific for a transporter, we could demonstrate the functional expression of OCT2, P-glycoprotein, MRP4, BCRP and OAT1 (in ciPTEC-OAT1) and OAT3 (in ciPTEC-OAT3). The transport of fluorescent probes could be inhibited using specific inhibitors or competitors.
Unlimited proliferation from cryo stocks
Just as any cell line, ciPTEC can be stored in liquid nitrogen to create your own stock. Upon thawing, ciPTEC will proliferate at 33°C and maintain it’s characteristics.
Suitable for drug-drug interaction studies
The transport of specific fluorescent probes could be inhibited using specific inhibitors or competitors, which can also be used to study drug-drug-interactions.
Biomarker expression upon toxicant exposure
In screening assays developed by Cell4Pharma, (non-)specific biomarkers for renal toxicity could be demonstrated upon toxicant exposure, including HO-1, miRNAs and LDH release.
Compatible with high throughput screening
Cell4Pharma’s ciPTEC can be cultured in 96 and 384 well plates. Together with it’s unlimited proliferation capacity allow high throughput screening.
Human origin
ciPTEC was originally derived from a human donor (female). The human origin results in the high predictivity for human renal adverse events and the versatile use of ciPTEC in the fields of pharmacology, nephrology, physiology and human pathology.
Used in 60+ peer-reviewed scientific publication
More than 10 years of research in world-wide academic and industry collaborations have lead to 60+ peer-reviewed scientific publications in the fields of pharmacology, nephrology, physiology and human pathology.
Industry Benefits
Low inter-laboratory
variations
Compatible with high-throughput screening equipment
Easy to use following standard culturing protocols
Expression of specific biomarkers for renal toxicity upon toxicant exposure
Reduction of animal experiments upon full validation
Applicable in Organ-on-a-Chip devices to mimic 3D tubular structures
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Partner Program:
Align our strengths to deliver more for your clients. Contact us to discuss partnership opportunities.
Advance training
– Extensive training for your organization
Customer Referrals and Services
– We refer sponsors in your geography and clinical expertise area
Expert Support
– Scientific support
– Sales Support
Product Input
– We value your input and take your scientific, customer and functionality needs into consideration in further development of ciPTEC.
Testimonials
Because the cell line is robust in culture, the research leads to reliable results. As far as I am concerned, there is currently no kidney epithelial cell line available with an equally good predictive value for the human kidney.
The ciPTEC mimics primary renal cells, but you can culture them for a long time without losing their properties. The expression of transporters gives a more accurate response to drugs and toxins. The cells grow fast and are easy to handle.
ciPTEC is a well-accepted in vitro model to screen compounds for their DIKI potential.