AssaysStudio Bolo2020-09-30T21:14:49+01:00 Standard culturing protocol, including freeze/thawing. available upon request: Request OAT function in ciPTEC: Fluorescein uptake (96well format) Nieskens et al. AAPSJ 2016 OCT2 function in ciPTEC: 4-Di-1-ASP transport assay Schophuizen et al. Pflugers Arch 2013 P-glycoprotein functionality in ciPTEC: Calcein-AM transport assay Caetano-Pinto et al. Mol Pharm 2016 MRP4 functionality in ciPTEC: CMFDA transporter assay Caetano-Pinto et al. Mol Pharm 2016 BCRP functionality in ciPTEC: Hoechst 33342 transporter assay Caetano-Pinto et al. Mol Pharm 2016 Receptor-mediated endocytosis: albumin and alpha-1-microglobulin uptake assay Janssen et al. Mol Ther Nucleic Acids 2019 Biomarker for toxicity: intracellular ATP levels combined lactate dehydrogenase leakage Vriend et al. Biol Chem 2019 Biomarker for toxicity: lactate dehydrogenase measurement Vormann et al. AAPS 2018 Biomarker for toxicity: MTT viability assay Nieskens et al. Drug Metab Dispos 2018 Biomarker for toxicity: WST-8 viability assay Suter-Dick et al. AAPS 2018 Biomarker for toxicity: NAG analysis in supernatant Suter-Dick et al. AAPS 2018 Biomarker for toxicity: detection of miRNA’s in medium Suter-Dick et al. AAPS 2018 Biomarker for toxicity: multi-parametric high content screening assay Sjögren et al Arch Toxicol 2018 Gene expression analysis in ciPTEC: mRNA isolation, cDNA synthesis and qPCR Nieskens et al. Drug Metab Dispos 2018 Kidney-on-a-chip using ciPTEC Vormann et al. AAPS 2018
