Assays

ciPTEC based
assays

Standard culturing protocol, including freeze/thawing.
available upon request:

Request

OAT function in ciPTEC: Fluorescein uptake (96well format)

Nieskens et al. AAPSJ 2016

OCT2 function in ciPTEC: 4-Di-1-ASP transport assay

Schophuizen et al. Pflugers Arch 2013

P-glycoprotein functionality in ciPTEC: Calcein-AM transport assay

Caetano-Pinto et al. Mol Pharm 2016

MRP4 functionality in ciPTEC: CMFDA transporter assay

Caetano-Pinto et al. Mol Pharm 2016

BCRP functionality in ciPTEC: Hoechst 33342 transporter assay

Caetano-Pinto et al. Mol Pharm 2016

Receptor-mediated endocytosis: albumin and alpha-1-microglobulin uptake assay

Janssen et al. Mol Ther Nucleic Acids 2019

Biomarker for toxicity: intracellular ATP levels combined lactate dehydrogenase leakage

Vriend et al. Biol Chem 2019

Biomarker for toxicity: lactate dehydrogenase measurement

Vormann et al. AAPS 2018

Biomarker for toxicity: MTT viability assay

Nieskens et al. Drug Metab Dispos 2018

Biomarker for toxicity: WST-8 viability assay

Suter-Dick et al. AAPS 2018

Biomarker for toxicity: NAG analysis in supernatant

Suter-Dick et al. AAPS 2018

Biomarker for toxicity: detection of miRNA’s in medium

Suter-Dick et al. AAPS 2018

Biomarker for toxicity: multi-parametric high content screening assay

Sjögren et al Arch Toxicol 2018

Gene expression analysis in ciPTEC: mRNA isolation, cDNA synthesis and qPCR

Nieskens et al. Drug Metab Dispos 2018

Kidney-on-a-chip using ciPTEC

Vormann et al. AAPS 2018